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Emil-Ramann-Str. 4， complete sequence.ACCESSION .VERSION.KEYWORDS .SOURCEsynthetic DNA construct ORGANISM synthetic DNA constructREFERENCE 1 (bases 1 to 6798) AUTHORS Heinze S， Ludwig C， Technical University ofMunich， Emil-Ramann-Str. 4， Freising， Liebl W， Heinzlmeir S， Germany"COMMENTSGRef: number: 3; type: "Journal Article"COMMENT##Assembly-Data-START##Sequencing Technology :: Sanger dideoxy sequencing##Assembly-Data-END##FEATURESLocation/Qualifierssource1..6798/mol_type="other DNA"/organism="synthetic DNA construct"rep_origincomplement(79..667)/direction=LEFT/label=ori/note="high-copy-number ColE1/pMB1/pBR322/pUC origin ofreplication"CDScomplement(841..1698)/label=AmpR/note="beta-lactamase"promotercomplement(1699..1803)/label=AmpR promoterCDScomplement(1832..2116)/codon_start=1/gene="traJ"/product="TraJ"/label=traJ/protein_id="AXF54495.1"/translation="MADETKPTRKGSPPIKVYCLPDERRAIEEKAAAAGMSLSAYLLAVGQGYKITGVVDYEHVRELARINGDLGQQKAHYVNHHPNQVFWGRGAVKH"genecomplement(1832..2116)/gene="traJ"/label=traJoriTcomplement(2149..2258)/direction=LEFT/label=oriT/note="incP origin of transfer"CDScomplement(2515..3276)/codon_start=1/gene="kanR"/product="KanR"/label=kanR/note="Kanamycin Resistance"/protein_id="AXF54496.1"/translation="MNGPIIMTREERMKIVHEIKERILDKYGDDVKAIGVYGSLGRQTDGPYSDIEMMCVMSTEEAEFSHEWTTGEWKVEVNFDSEEILLDYASQVESDWPLTHGQFFSILPIYDSGGYLEKVYQTAKSVEAQTFHDAICALIVEELFEYAGKWRNIRVQGPTTFLPSLTVQVAMAGAMLIGLHHRICYTTSASVLTEAVKQSDLPSGYDHLCQFVMSGQLSDSEKLLESLENFWNGIQEWTERHGYIVDVSKRIPF"genecomplement(2515..3276)/gene="kanR"/label=kanRCDScomplement(3448..4449)/label=repB/note="RepB replication protein"regulatory5069..5124/label=P43 promoter from B. subtilis/note="P43 promoter from B. subtilis"/regulatory_class="promoter"CDS5175..5258/codon_start=1/gene="SPLipB-Cel8A-His6"/product="LipB signal peptide"/label=SPLipB-Cel8A-His6/protein_id="AXF54497.1"/translation="MKKVLMAFIICLSLILSVLAAPPSGAKA"CDS6618..6635/label=6xHis/note="6xHis affinity tag" ， Kornberger P. TITLEEfficient secretory heterologous enzyme production in the novel hostPaenibacillus polymyxa by usage of its promoter sequences JOURNAL UnpublishedREFERENCE 2 (bases 1 to 6798) AUTHORS Heinze S， Schwarz WH，GermanyREFERENCE 3 (bases 1 to 6798) TITLEDirect SubmissionREFERENCE 4 (bases 1 to 6798) AUTHORS . TITLEDirect SubmissionCOMMENTSGRef: number: 1; type: "Journal Article"; journalName:"Unpublished"COMMENTSGRef: number: 2; type: "Journal Article"; journalName: "Submitted(09-MAY-2018) Department for Microbiology， LOCUS40924_340176798 bp DNAcircular SYN 18-DEC-2018DEFINITION Shuttle vector pP43-cel8A， TechnicalUniversity of Munich， Zimmermann K， Zimmermann K， ZverlovVV， Heinzlmeir S。
Liebl W， Ludwig C， ZverlovVV。
Schwarz WH， Freising，。
Kornberger P. TITLEDirect Submission JOURNAL Submitted (09-MAY-2018) Department for Microbiology， Bavaria 85354， Bavaria 85354。